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Chapitre d'ouvrage

Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

Abstract : Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.
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Soumis le : lundi 17 février 2020 - 14:48:22
Dernière modification le : vendredi 4 mars 2022 - 14:02:01




Ines Carqueijeiro, Liuda Sepúlveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, et al.. Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies. In: Pereira C. (eds) Plant Vacuolar Trafficking. Methods in Molecular Biology, vol 1789. Humana Press, New York, NY, 1789, pp.33-54, 2018, ⟨10.1007/978-1-4939-7856-4_4⟩. ⟨hal-02481460⟩



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