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A high-quality sequence of Rosa chinensis to elucidate genome structure and ornamental traits

Laurence Hibrand-Saint Oyant 1 Tom Ruttink 2 Latifa Hamama 1 Ilya Kirov 3 Deepika Lakhwani 1 Ning-Ning Zhou 1 Peter Bourke 4 Nicolas Daccord 1 Leen Leus 2 Dietmar Schulz 5 Henri van de Geest Thamara Hesselink Katrijn van Laere Sandrine Balzergue 1 Tatiana Thouroude 1 Annie Chastellier 1 Julien Jeauffre 1 Linda Voisine 1 Sylvain Gaillard 1 Theo Borm 4 Paul Arens 4 Roeland Voorrips Chris Maliepaard Enzo Neu Marcus Linde Marie-Christine Le Paslier Aurelie Berard 6 Remi Bounon 7 Jeremy Clotault 1 Nathalie Choisne 8 Hadi Quesneville 8 Koji Kawamura Sébastien Aubourg 1 Soulaiman Sakr 1 Rene Smulder Elio Schijlen Etienne Bucher 1 Thomas Debener Jan de Riek Fabrice Foucher 1
1 IRHS - Institut de Recherche en Horticulture et Semences
2 ILVO - Research Institute for Agricultural, Fisheries and Food
3 Russian State Agrarian University - Moscow Agricultural Academy named after K.A. Timiryazev
4 Wageningen University
5 LUH - Leibniz Universität Hannover [Hannover]
6 UR Etude du Polymorphisme des Génomes végétaux
7 CEA - Commissariat à l'énergie atomique et aux énergies alternatives
8 URGI - Unité de Recherche Génomique Info
Abstract : Rose is the worlds most important ornamental plant with economic, cultural and symbolic value. Roses are cultivated worldwide and sold as garden roses, cut flowers and potted plants. Rose has a complex genome with high heterozygosity and various ploidy levels. Our objectives were (i) to develop the first high-quality reference genome sequence for the genus Rosa by sequencing a doubled haploid, combining long and short read sequencing, and anchoring to a high-density genetic map and (ii) to study the genome structure and the genetic basis of major ornamental traits. We produced a haploid rose line from R. chinensis "Old Blush" and generated the first rose genome sequence at the pseudo-molecule scale (512 Mbp with N50 of 3.4 Mb and L75 of 97). The sequence was validated using high-density diploid and tetraploid genetic maps. We delineated hallmark chromosomal features including the pericentromeric regions through annotation of TE families and positioned centromeric repeats using FISH. Genetic diversity was analysed by resequencing eight Rosa species. Combining genetic and genomic approaches, we identified potential genetic regulators of key ornamental traits, including prickle density and number of flower petals. A rose APETALA2 homologue is proposed to be the major regulator of petals number in rose. This reference sequence is an important resource for studying polyploidisation, meiosis and developmental processes as we demonstrated for flower and prickle development. This reference sequence will also accelerate breeding through the development of molecular markers linked to traits, the identification of the genes underlying them and the exploitation of synteny across Rosaceae.
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https://hal.univ-angers.fr/hal-02516576
Contributeur : Okina Université d'Angers <>
Soumis le : mardi 24 mars 2020 - 01:27:59
Dernière modification le : lundi 13 septembre 2021 - 14:46:02

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UNIV-ANGERS | INRAE | AGROCAMPUS-OUEST | AGROCAMPUS-OUEST-UMR-IRHS | INRA | UNAM | IRHS | CEA

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Laurence Hibrand-Saint Oyant, Tom Ruttink, Latifa Hamama, Ilya Kirov, Deepika Lakhwani, et al.. A high-quality sequence of Rosa chinensis to elucidate genome structure and ornamental traits. Nature Plants, Nature Publishing Group, 2018, pp.473-484. ⟨10.1101/254102⟩. ⟨hal-02516576⟩

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