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Article dans une revue

Niches and routes of transmission of Xanthomonas citri pv. fuscans to bean seeds

Abstract :

Aims

Seeds are vectors of a diversified microbiota including plant pathogens. To better understand transmission of common bacterial blight (CBB) agents to bean seeds, we analyzed the role of non-pathogenic xanthomonads on seed transmission efficiency and investigated the location of Xanthomonas citri pv. fuscans (Xcf) into seeds and plantlets.

Methods

Competition between CBB and NP strains was initially assessed in vitro and then extended in planta to monitor the impact of co-inoculation on Xcf seed transmission. Moreover, location of Xcf strains in seeds and seedlings was visualized using a combination of gfp-tagged strain and DOPE-FISH/CSLM.

Results

Whereas CBB agent growth was inhibited in vitro by some seed-borne non-pathogenic xanthomonads strains, these strains did not transmit efficiently to seed through floral pathway and did not affect Xcf seed transmission. Xcf cells were observed entering seed through vascular elements and parenchyma of funiculus, but also micropyle and testa. Xcf cells were observed, moreover, among other bacteria on radicle surfaces, especially tip, in cotyledons, and plumules.

Conclusions

CBB agents are more efficient than non-pathogenic xanthomonads in using the floral route to colonize seeds. CBB agents are located within different niches in the seed tissues up to the embryonic axis.

Type de document :
Article dans une revue
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https://hal.univ-angers.fr/hal-02516672
Contributeur : Okina Université d'Angers <>
Soumis le : mardi 24 mars 2020 - 03:32:46
Dernière modification le : lundi 6 juillet 2020 - 15:40:10

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Citation

Armelle Darrasse, Matthieu Barret, Sophie Cesbron, Stéphane Compant, Marie-Agnès Jacques. Niches and routes of transmission of Xanthomonas citri pv. fuscans to bean seeds. Plant and Soil, Springer Verlag, 2018, 422 (1-2), pp.115-128. ⟨10.1007/s11104-017-3329-3⟩. ⟨hal-02516672⟩

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