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Communication dans un congrès

Laser Desorption Ionization and Related Methods for the Detection of Small Molecules

Abstract : In recent years metabolomic profiling and dereplication have become important tools in the field of natural products’ (NPs) research and quality control. These experiments are mainly based on methods such as high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) as well as 1H and 13C nuclear magnetic resonance spectroscopy (NMR) (El-Elimat, T., et al. J. Nat. Prod. 2013, 76, 1709–1716; Williams, R.B., et al. Org. Biomol. Chem. 2015, 39, 9957–9962). On the other hand matrix assisted matrix laser desorption ionization (MALDI) and matrix free laser desorption ionization (LDI) are hardly discussed in this context. This lecture will highlight some recent advances in both methods such as the development of selective MALDI matrices for the detection of alkaloids in complex mixtures but also the matrix free LDI of NPs. Many NPs exhibit close structural similarities to MALDI matrices and can be directly ionized by LDI (Schinkovitz, A., et al. J. Mass Spectrom. 2015, 50 , 270–274 ; Le-Pogam, P., et al. Anal. Chem. 2015, 87, 10421–10428). The effect is observed for a large variety of different compounds belonging to various chemical families, such as flavonoids, coumarins, tocopherols, and alkaloids, some of which could even be detected from crude extracts. Eventually the concept of selective signal enhancement by multi-wavelength laser irradiation targeting absorption maxima of selected analytes will be discussed
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Communication dans un congrès
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Contributeur : Okina Université d'Angers <>
Soumis le : vendredi 27 mars 2020 - 12:21:16
Dernière modification le : vendredi 5 juin 2020 - 14:44:17


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Andreas Schinkovitz, Ali Jaber, Séverine Boisard, Annika Koch, Klaus Dreisewerd, et al.. Laser Desorption Ionization and Related Methods for the Detection of Small Molecules. 24th GP2A Conference (Groupement de Pharmacochimist de l'Arc Atlantique), Aug 2016, Angers, France. ⟨10.3390/ph10010017⟩. ⟨hal-02521289⟩



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