In vitro separation of chimeral pears into their component genotypes, Euphytica, vol.48, pp.189-196, 1990. ,
Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system, Plant Methods, vol.9, p.39, 2013. ,
The draft genome sequence of European pear (Pyrus communis L, PLoS One, vol.9, p.92644, 2014. ,
A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants, Hortic. Res, vol.5, p.13, 2018. ,
Multiple sequence alignment with hierarchical clustering, Nucl. Acids Res, vol.16, 1988. ,
High-quality de novo assembly of the apple genome and methylome dynamics of early fruit development, Nat. Genet, vol.49, pp.1099-1106, 2017. ,
URL : https://hal.archives-ouvertes.fr/hal-01602554
CRISPR/Cas9 editing of carotenoid genes in tomato, Transgenic Res, vol.27, pp.367-378, 2018. ,
Efficient CRISPR/Cas9-mediated targeted mutagenesis in Populus in the first generation, Sci. Rep, vol.5, p.12217, 2015. ,
Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana, Plant J, vol.79, pp.348-359, 2014. ,
Transient expression of CRISPR/Cas9 machinery targeting TcNPR3 enhances defense response in Theobroma cacao, Front. Plant Sci, vol.9, p.268, 2018. ,
The MdTFL1 gene of apple (Malus x domestica Borkh.) reduces vegetative growth and generation time, Tree Physiol, vol.32, pp.1288-1301, 2012. ,
Development of a transgenic early flowering pear (Pyrus communis L.) genotype by RNAi silencing of PcTFL1-1 and PcTFL1-2, Planta, vol.235, pp.1239-1251, 2012. ,
URL : https://hal.archives-ouvertes.fr/hal-01209925
Microprep protocol for extraction of DNA from tomato and other herbaceous plants, Plant Mol. Biol. Rep, vol.13, pp.207-209, 1995. ,
Evaluation of off-target and on-target scoring algorithms and integration into the guide RNA selection tool CRISPOR, Genome Biol, vol.17, p.148, 2016. ,
URL : https://hal.archives-ouvertes.fr/hal-01346049
DNA cloning using in vitro site-specific recombination, Genome Res, vol.10, pp.1788-1795, 2000. ,
New Agrobacterium helper plasmids for gene transfer to plants, Transgenic Res, vol.2, pp.208-218, 1993. ,
Genetic transformation of apple (Malus pumila Mill) using a disarmed Ti-binary vector, Plant Cell Rep, vol.7, pp.658-661, 1989. ,
Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating Xcc pthA4:dCsLOB1.3 infection, Plant Biotechnol. J, vol.14, pp.1291-1301, 2016. ,
, , 2017.
, Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker, Plant Biotechnol. J, vol.15, pp.817-823
Targeted genome editing of sweet orange using Cas9/sgRNA, PLoS One, vol.9, p.93806, 2014. ,
Concerns regarding 'off-target' activity of genome editing endonucleases, Plant Pysiol. Biochem, vol.131, pp.22-30, 2018. ,
Gateway vectors for Agrobacteriummediated plant transformation, Trends Plant Sci, vol.7, pp.2251-2254, 2002. ,
Antisense expression of MdTFL1, a TFL1-like gene, reduces the juvenile phase in apple, J. Am. Soc. Hort. Sci, vol.131, pp.74-81, 2006. ,
Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.), Plant Cell Rep, vol.25, pp.99-105, 1991. ,
Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9, Nat. Biotechnol, vol.31, pp.688-691, 2013. ,
CRISPR/Cas9 platforms for genome editing in plants: developments and applications, Mol. Plant, vol.9, pp.961-974, 2016. ,
, , 2016.
, DNA-free genetically edited grapevine and apple protoplast using CRISPR/Cas9 ribonucleoproteins, Front. Plant Sci, vol.7, p.194
Pear, Compendium of Transgenic Crop Plants: Transgenic Temperate Fruits and Nuts, pp.53-77, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-01608142
Apple, Compendium of Transgenic Crop Plants: Transgenic Temperate Fruits and Nuts, pp.1-51, 2008. ,
URL : https://hal.archives-ouvertes.fr/hal-01210015
Touchdown method for high-performance sequencing of polymerase chain reaction products, Anal. Biochem, vol.315, pp.270-272, 2003. ,
Amplification of plant U3 and U6 snRNA gene sequences using primers specific for an upstream promoter element and conserved intragenic regions, Nucleic Acids Res, vol.18, pp.3459-3466, 1990. ,
Four TFL1/CEN-like genes on distinct linkage groups show different expression patterns to regulate vegetative and reproductive development in apple (Malus x domestica Borkh, Plant Cell Physiol, vol.50, pp.394-412, 2009. ,
Efficient Agrobacterium-mediated transformation and recovery of transgenic plants from pear (Pyrus communis L.), Plant Cell Rep, vol.16, pp.245-249, 1996. ,
A revised medium for rapid growth and bioassays with tobacco tissue culture, Physiol. Plant, vol.15, pp.473-497, 1962. ,
CRIPSR/Cas9-mediated targeted mutagenesis in grape, PLoS One, vol.12, p.177966, 2017. ,
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonucleases, Nat. Biotechnol, vol.31, pp.691-693, 2013. ,
Efficient genome editing in apple using a CRISPR/Cas9 system, Sci. Rep, vol.6, p.31481, 2016. ,
Engineering cankerresistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus, Plant Biotechnol. J, vol.15, pp.1509-1519, 2017. ,
Disruption of phytoene desaturase gene results in albino and dwarf phenotypes in Arabidopsis by impairing chlorophyll, carotenoid and gibberellin biosynthesis, Cell Res, vol.17, pp.471-482, 2007. ,
, , 2016.
, CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay, Vitis vinifera L.). Sci. Rep, vol.6, p.32289
Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectors, Plant Methods, vol.7, p.15, 2011. ,
A mutation in the Arabidopsis TFL 1 gene affects inflorescence meristem development, Plant Cell, vol.3, pp.877-892, 1991. ,
The ternary transformation system: constitutive virG on a compatible plasmid dramatically increases Agrobacterium-mediated plant transformation, Plant Mol. Biol, vol.43, pp.495-502, 2000. ,
The genome of the domesticated apple (Malus × domestica Borkh.), Nat. Genet, vol.42, pp.833-839, 2010. ,
An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus, Plant J, vol.33, pp.949-956, 2003. ,
CRIPSR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation, Plant Biotechnol. J, vol.16, pp.844-855, 2018. ,
Optimized paired-sgRNA/Cas9 cloning and expression cassette triggers high-efficiency multiplex genome editing in kiwifruit, Plant Biotechnol. J, vol.16, pp.1424-1433, 2018. ,
Heat mediated silencing of MdTFL1 genes in apple, Plant Cell Tis. Org. Cult, vol.123, pp.511-521, 2015. ,
The genome of pear (Pyrus bretschneideri Redh.), Genome Res, vol.23, pp.396-408, 2013. ,