A. Patel, M. Patel, X. Yang, and A. Mitra, Recent Advances in Protein and Peptide Drug Delivery: A Special Emphasis on Polymeric Nanoparticles, Protein & Peptide Letters, vol.21, issue.11, pp.1102-1120, 2014.

R. Ghasemi, M. Abdollahi, E. Emamgholi-zadeh, K. Khodabakhshi, A. Badeli et al., mPEG-PLA and PLA-PEG-PLA nanoparticles as new carriers for delivery of recombinant human Growth Hormone (rhGH), Scientific Reports, vol.8, issue.1, p.433, 2018.

S. Hosseinkhani and P. Mpeg-pla, PLA nanoparticles as new carriers for 434 delivery of recombinant human Growth Hormone (rhGH), vol.8, pp.1-13, 2018.

S. P. Dipak, M. P. Kosloski, and S. V. Balu-iyer, Delivery of Therapeutic Proteins, J Pharm Sci, vol.437, pp.2557-2575, 2010.

R. F. Pagels and R. K. Prud'homme, Polymeric nanoparticles and microparticles for the delivery of peptides, biologics, and soluble therapeutics, Journal of Controlled Release, vol.219, pp.519-535, 2015.

S. Mohammadi-samani and B. Taghipour, PLGA micro and nanoparticles in delivery of peptides and proteins; problems and approaches, Pharmaceutical Development and Technology, vol.20, issue.4, pp.385-393, 2014.

S. Fredenberg, M. Reslow, and A. Axelsson, Measurement of Protein Diffusion Through Poly(d,l-Lactide-Co-Glycolide), Pharmaceutical Development and Technology, vol.10, issue.2, pp.299-307, 2005.

T. Kissel, Y. Li, and F. Unger, ABA-triblock copolymers from biodegradable polyester A-blocks and hydrophilic poly(ethylene oxide) B-blocks as a candidate for in situ forming hydrogel delivery systems for proteins, Advanced Drug Delivery Reviews, vol.54, issue.1, pp.99-134, 2002.

, , pp.244-251

K. Zhang, X. Tang, J. Zhang, W. Lu, X. Lin et al., PEG?PLGA copolymers: Their structure and structure-influenced drug delivery applications, Journal of Controlled Release, vol.183, pp.77-86, 2014.

S. Feng, L. Nie, P. Zou, and J. Suo, Effects of drug and polymer molecular weight on drug release from PLGA-mPEG microspheres, Journal of Applied Polymer Science, vol.132, issue.6, pp.n/a-n/a, 2014.

Y. Wei, Y. Wang, H. Zhang, W. Zhou, and G. Ma, A novel strategy for the preparation of porous microspheres and its application in peptide drug loading, Journal of Colloid and Interface Science, vol.478, pp.46-53, 2016.

B. Patel, V. Gupta, and F. Ahsan, PEG?PLGA based large porous particles for pulmonary delivery of a highly soluble drug, low molecular weight heparin, Journal of Controlled Release, vol.162, issue.2, pp.310-320, 2012.

V. Tran, J. Karam, X. Garric, J. Coudane, J. Benoît et al., Protein-loaded PLGA?PEG?PLGA microspheres: A tool for cell therapy, European Journal of Pharmaceutical Sciences, vol.45, issue.1-2, pp.128-137, 2012.
URL : https://hal.archives-ouvertes.fr/hal-00681285

. Venier-julienne, Protein-loaded PLGA-PEG-PLGA microspheres: A tool for cell therapy, p.465
URL : https://hal.archives-ouvertes.fr/hal-00681285

, Eur. J. Pharm. Sci, vol.45, pp.128-137, 2012.

M. Le, F. Violet, C. Paniagua, X. Garric, and M. Venier-julienne, Penta-block copolymer microspheres: Impact of polymer characteristics and process parameters on protein release, International Journal of Pharmaceutics, vol.535, issue.1-2, pp.428-437, 2018.
URL : https://hal.archives-ouvertes.fr/hal-02616041

, Int. J. Pharm, vol.535, pp.428-437, 2018.

A. Giteau, M. Venier-julienne, S. Marchal, J. Courthaudon, M. Sergent et al., , p.470

J. Menei, J. Verdier, and . Benoit, Reversible protein precipitation to ensure stability 471 during encapsulation within PLGA microspheres, Eur. J. Pharm. Biopharm, vol.70, pp.472-127, 2008.

A. Paillard-giteau, V. T. Tran, O. Thomas, X. Garric, J. Coudane et al., Effect of various additives and polymers on lysozyme release from PLGA microspheres prepared by an s/o/w emulsion technique, European Journal of Pharmaceutics and Biopharmaceutics, vol.75, issue.2, pp.128-136, 2010.
URL : https://hal.archives-ouvertes.fr/hal-00626154

J. P. Benoît, C. N. Montero-menei, and M. C. Venier-julienne, Effect of various additives and 475 polymers on lysozyme release from PLGA microspheres prepared by an s/o/w emulsion 476 technique, Eur. J. Pharm. Biopharm, vol.75, pp.128-136, 2010.

J. Buske, C. König, S. Bassarab, A. Lamprecht, S. Mühlau et al., Influence of PEG in PEG?PLGA microspheres on particle properties and protein release, European Journal of Pharmaceutics and Biopharmaceutics, vol.81, issue.1, pp.57-63, 2012.

. Biopharm, , vol.81, pp.57-63, 2012.

M. Morille, T. Van-thanh, X. Garric, J. Cayon, J. Coudane et al., New PLGA?P188?PLGA matrix enhances TGF-?3 release from pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal stem cells, Journal of Controlled Release, vol.170, issue.1, pp.99-110, 2013.
URL : https://hal.archives-ouvertes.fr/hal-00881128

C. N. Julienne and . Montero-menei, New PLGA-P188-PLGA matrix enhances TGF-beta3

R. T. Liggins and H. M. Burt, Paclitaxel-loaded poly(l-lactic acid) microspheres 3: blending low and high molecular weight polymers to control morphology and drug release, International Journal of Pharmaceutics, vol.282, issue.1-2, pp.61-71, 2004.

. Pharm, , vol.282, pp.61-71, 2004.

M. V. Balashanmugam, S. Nagarethinam, H. Jagani, V. R. Josyula, A. Alrohaimi et al., Preparation and Characterization of Novel PBAE/PLGA Polymer Blend Microparticles for DNA Vaccine Delivery, The Scientific World Journal, vol.2014, pp.1-9, 2014.

J. Liu, S. Li, G. Li, X. Li, C. Yu et al., Highly bioactive, bevacizumab-loaded, sustained-release PLGA/PCADK microspheres for intravitreal therapy in ocular diseases, International Journal of Pharmaceutics, vol.563, pp.228-236, 2019.

B. Gu and D. J. Burgess, Prediction of dexamethasone release from PLGA microspheres prepared with polymer blends using a design of experiment approach, International Journal of Pharmaceutics, vol.495, issue.1, pp.393-403, 2015.

C. F. Van-der-walle and O. Olejnik, An Overview of the Field of Peptide and Protein Delivery, Peptide and Protein Delivery, pp.1-22, 2011.

Q. M. ,

C. R. Rowe and J. P. Sheskey, Handbook of Pharmaceutical Excipients, 2009.

A. Aubert?pouëssel, D. C. Bibby, M. Venier?julienne, F. Hindré, and J. P. Benoît, A novel in 505 vitro delivery system for assessing the biological integrity of protein upon release from 506 PLGA microspheres, Pharmaceutical Research, vol.19, issue.7, pp.1046-1051, 2002.

A. Aubert?pouëssel, D. C. Bibby, M. Venier?julienne, F. Hindré, and J. Benoît, Pharmaceutical Research, vol.19, issue.7, pp.1046-1051, 2002.

M. O. Omelczuk and J. W. Mcginity, The Influence of Polymer Glass Transition Temperature 509 and Molecular Weight on Drug Release from Tablets Containing Poly, p.510

, International Journal of Research in Pharmacy and Pharmaceutical Sciences, Pharm. Res. An Off. J. Am. Assoc. Pharm. Sci, vol.9, pp.26-32

M. O. Omelczuk and J. W. Mcginity, Pharmaceutical Research, vol.09, issue.1, pp.26-32, 1992.

A. Santoveña, C. Alvarez-lorenzo, A. Concheiro, M. Llabrés, and J. B. Fariña, Structural properties of biodegradable polyesters and rheological behaviour of their dispersions and films, Journal of Biomaterials Science, Polymer Edition, vol.16, issue.5, pp.629-641, 2005.

J. J. Rouse, F. Mohamed, and C. F. Van-der-walle, Physical ageing and thermal analysis of PLGA microspheres encapsulating protein or DNA, International Journal of Pharmaceutics, vol.339, issue.1-2, pp.112-120, 2007.

K. Vay, W. Frieß, and S. Scheler, A detailed view of microparticle formation by in-process monitoring of the glass transition temperature, European Journal of Pharmaceutics and Biopharmaceutics, vol.81, issue.2, pp.399-408, 2012.

S. Fredenberg, M. Wahlgren, M. Reslow, and A. Axelsson, The mechanisms of drug release in poly(lactic-co-glycolic acid)-based drug delivery systems?A review, International Journal of Pharmaceutics, vol.415, issue.1-2, pp.34-52, 2011.

X. Wang, S. S. Venkatraman, F. Y. Boey, J. S. Loo, and L. P. Tan, Controlled release of sirolimus from a multilayered PLGA stent matrix, Biomaterials, vol.27, issue.32, pp.5588-5595, 2006.

N. Passerini and D. Q. Craig, An investigation into the effects of residual water on the glass transition temperature of polylactide microspheres using modulated temperature DSC, Journal of Controlled Release, vol.73, issue.1, pp.111-115, 2001.

N. I. Li, K. W. Anderson, and P. P. Deluca, Kinetic and thermodynamic modeling of the formation of polymeric microspheres using solvent extraction/evaporation method, Journal of Controlled Release, vol.37, issue.3, pp.187-198, 1995.

, Control. Release, vol.37, pp.77-78, 1995.

N. I. Li, K. W. Anderson, R. C. Mehta, and P. P. Deluca, Prediction of solvent removal profile and effect on properties for peptide-loaded PLGA microspheres prepared by solvent extraction/ evaporation method, Journal of Controlled Release, vol.37, issue.3, pp.199-214, 1995.

, , pp.76-79

X. Fu, Q. Ping, and Y. Gao, Effects of formulation factors on encapsulation efficiency and release behaviourin vitroof huperzine A-PLGA microspheres, Journal of Microencapsulation, vol.22, issue.7, pp.705-714, 2005.

X. Luan, M. Skupin, J. Siepmann, and R. Bodmeier, Key parameters affecting the initial release (burst) and encapsulation efficiency of peptide-containing poly(lactide-co-glycolide) microparticles, International Journal of Pharmaceutics, vol.324, issue.2, pp.168-175, 2006.

H. Park, D. H. Ha, E. H. Ha, J. S. Kim, M. S. Kim et al., Effect of Stabilizers on Encapsulation Efficiency and Release Behavior of Exenatide-Loaded PLGA Microsphere Prepared by the W/O/W Solvent Evaporation Method, Pharmaceutics, vol.11, issue.12, p.627, 2019.

H. Park, D. Ha, E. Ha, J. Kim, M. Kim et al., Effect of Stabilizers on Encapsulation Efficiency and Release Behavior of Exenatide-Loaded PLGA Microsphere Prepared by the W/O/W Solvent Evaporation Method, Pharmaceutics, vol.11, issue.12, p.627, 2019.

J. Siepmann, N. Faisant, J. Akiki, J. Richard, and J. P. Benoit, Effect of the size of biodegradable microparticles on drug release: experiment and theory, Journal of Controlled Release, vol.96, issue.1, pp.123-134, 2004.

D. Klose, F. Siepmann, K. Elkharraz, S. Krenzlin, and J. Siepmann, How porosity and size affect the drug release mechanisms from PLGA-based microparticles, International Journal of Pharmaceutics, vol.314, issue.2, pp.198-206, 2006.

Y. Boukari, O. Qutachi, D. J. Scurr, A. P. Morris, S. W. Doughty et al., A dual-application poly (dl-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering, Journal of Biomaterials Science, Polymer Edition, vol.28, issue.16, pp.1966-1983, 2017.

Y. Boukari, D. J. Scurr, O. Qutachi, A. P. Morris, S. W. Doughty et al., Physicomechanical properties of sintered scaffolds formed from porous and protein-loaded poly(DL-lactic-co-glycolic acid) microspheres for potential use in bone tissue engineering, Journal of Biomaterials Science, Polymer Edition, vol.26, issue.12, pp.796-811, 2015.

E. D?aurizio, C. F. Van-nostrum, M. J. Van-steenbergen, P. Sozio, F. Siepmann et al., Preparation and characterization of poly(lactic-co-glycolic acid) microspheres loaded with a labile antiparkinson prodrug, International Journal of Pharmaceutics, vol.409, issue.1-2, pp.289-296, 2011.

W. E. Siepmann, A. D. Hennink, and . Stefano, Preparation and characterization of poly(lactic-562 co-glycolic acid) microspheres loaded with a labile antiparkinson prodrug, Int. J. Pharm, vol.563, pp.289-296, 2011.

C. K. Sackett and B. Narasimhan, Mathematical modeling of polymer erosion: Consequences for drug delivery, International Journal of Pharmaceutics, vol.418, issue.1, pp.104-114, 2011.

A. N. Ford, D. W. Versypt, R. D. Pack, and . Braatz, Mathematical modeling of drug delivery from 568 autocatalytically degradable PLGA microspheres--a review, J. Control. Release, vol.165, pp.29-37, 2013.

D. Klose, F. Siepmann, K. Elkharraz, and J. Siepmann, PLGA-based drug delivery systems: Importance of the type of drug and device geometry, International Journal of Pharmaceutics, vol.354, issue.1-2, pp.95-103, 2008.

A. N. Ford, P. D. Versypt, D. W. Arendt, R. D. Pack, and . Braatz, Derivation of an Analytical 574 Solution to a Reaction-Diffusion Model for Autocatalytic Degradation and Erosion in 575 Polymer Microspheres, PLoS One, vol.10, p.135506, 2015.

A. N. Ford-versypt, P. D. Arendt, D. W. Pack, and R. D. Braatz, Derivation of an Analytical Solution to a Reaction-Diffusion Model for Autocatalytic Degradation and Erosion in Polymer Microspheres, PLOS ONE, vol.10, issue.8, p.e0135506, 2015.

C. Berkland, E. Pollauf, C. Raman, R. Silverman, K. (. Kim et al., Macromolecule Release from Monodisperse PLG Microspheres: Control of Release Rates and Investigation of Release Mechanism, Journal of Pharmaceutical Sciences, vol.96, issue.5, pp.1176-1191, 2007.

C. Zheng, J. Gao, W. Liang, H. Yu, and Y. Zhang, Poly(lactic-co-glycolic acid) Microspheres, Polymers in Drug Delivery, vol.60, pp.54-59, 2006.

X. Huang, N. Li, D. Wang, Y. Luo, Z. Wu et al.,

. Wu, Quantitative three-dimensional analysis of poly (lactic-co-glycolic acid) microsphere 586 using hard X-ray nano-tomography revealed correlation between structural parameters and 587 drug burst release, J. Pharm. Biomed. Anal, vol.112, pp.43-49, 2015.

X. Huang, N. Li, D. Wang, Y. Luo, Z. Wu et al., Quantitative three-dimensional analysis of poly (lactic-co-glycolic acid) microsphere using hard X-ray nano-tomography revealed correlation between structural parameters and drug burst release, Journal of Pharmaceutical and Biomedical Analysis, vol.112, pp.43-49, 2015.

T. Hickey, D. Kreutzer, D. J. Burgess, and F. Moussy, Dexamethasone/PLGA microspheres for continuous delivery of an anti-inflammatory drug for implantable medical devices, Biomaterials, vol.23, issue.7, pp.1649-1656, 2002.

, Biomaterials, vol.23, pp.291-296, 2002.

S. Duvvuri, K. G. Janoria, D. Pal, and A. K. Mitra, Controlled Delivery of Ganciclovir to the Retina with Drug-Loaded Poly(D,L-lactide-co-glycolide) (PLGA) Microspheres Dispersed in PLGA-PEG-PLGA Gel: A Novel Intravitreal Delivery System for the Treatment of Cytomegalovirus Retinitis, Journal of Ocular Pharmacology and Therapeutics, vol.23, issue.3, pp.264-274, 2007.

P. , Gel: a novel intravitreal delivery system for the treatment of 595 cytomegalovirus retinitis, J. Ocul. Pharmacol. Ther, vol.23, pp.264-274, 2007.