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Communication dans un congrès

Design of polyamines-grafted starches for nucleotide analogues delivery: in vitro evaluation of the anticancer activity

Abstract :

Nucleotide analogues are a therapeutic class really promising and currently used in clinic notably against viral infectious diseases and cancer.1 However, their therapeutic potential is often restricted by a poor stability in vivo, the induction of severe side effects and a limited passive intracellular diffusion due to their hydrophilicity.2 Polysaccharide-based polymers (e. g. starch) have considerable advantages including a lack of toxicity and absence of antigenicity.3–5 The aim of this study was to develop new cationic starches able to form complexes with nucleotide analogues: to protect them and increase their cell uptake. The material should demonstrate good biocompatibility and low cytotoxicity. Different oligoamines, (TREN, TEPA and spermine) were covalently grafted to starch : the resulting cationic starch derivatives were characterized (e.g. degree of modification) and compared in their properties to form polyplexes with ATP as a model nucleotide. Among the tested candidates, the formulation of starch-TEPA and ATP with a N/P ratio = 2 led to nanoparticles with a size of 429 nm, a PdI of 0.054 and a zeta potential of -9 mV. MTT and LDH assays on A549 cell line showed a low toxicity of this cationic starch. Confocal microscopy studies proved that the cell internalization was an incubation time and energy dependent process. Most important, starch-TEPA complexes with ddGTP (0.3 mg/mL) showed a significant biological activity on A549 cancer cells (> 90 %) compared to plain ddGTP (~ 21 %) at the same concentration, revealing a real promising system to deliver intracellularly nucleotide analogues.

Type de document :
Communication dans un congrès
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https://hal.univ-angers.fr/hal-02615958
Contributeur : Okina Université d'Angers <>
Soumis le : dimanche 24 mai 2020 - 01:53:40
Dernière modification le : dimanche 24 mai 2020 - 03:17:52

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  • HAL Id : hal-02615958, version 1
  • OKINA : ua15086

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Citation

Elise Lepeltier. Design of polyamines-grafted starches for nucleotide analogues delivery: in vitro evaluation of the anticancer activity. 24th Conference of the Groupement des Pharmacochimistes de l'Arc Atlantique (GP2A), 2016, Angers, France. ⟨hal-02615958⟩

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