Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Comparative whole corona fingerprinting and protein adsorption thermodynamics of PLGA and PCL nanoparticles in human serum

Abstract : Nanoparticles (NPs) based on biocompatible and biodegradable polymers such as poly(lactic-co-glycolic acid) (PLGA) and polycaprolactone (PCL) represent effective systems for systemic drug delivery. Upon injection into the blood circuit, the NP surface is rapidly modified due to adsorption of proteins that form a 'protein corona' (PC). The PC plays an important role in cellular targeting, uptake and NP bio-distribution. Hence, the study of interactions between NPs and serum proteins appears as key for biomedical applications and safety of NPs. In the present work, we report on the comparative protein fluorescence quenching extent, thermodynamics of protein binding and identification of proteins in the soft and hard corona layers of PLGA and PCL NPs. NPs were prepared via a single emulsion-solvent evaporation technique and characterized with respect to size, zeta potential, surface morphology and hydrophobicity. Protein fluorescence quenching experiments were performed against human serum albumin. The thermodynamics of serum protein binding onto the NPs was studied using iso-thermal titration calorimetry. Semi-quantitative analysis of proteins in the PC layers was conducted using gel electrophoresis and mass spectrometry using human serum. Our results demonstrated the influence of particle hydrophobicity on the thermodynamics of protein binding. Human serum proteins bind to a greater extent and with greater affinity to PCL NPs than PLGA NPs. Several proteins were detected in the hard and soft corona of the NPs, representing their unique proteome fingerprints. Some proteins were unique to the PCL NPs. We anticipate that our findings will assist with rational design of polymeric NPs for effective drug delivery applications.
Type de document :
Article dans une revue
Liste complète des métadonnées

Littérature citée [53 références]  Voir  Masquer  Télécharger

https://hal.univ-angers.fr/hal-02778503
Contributeur : David Canevet <>
Soumis le : jeudi 4 juin 2020 - 16:22:33
Dernière modification le : samedi 27 juin 2020 - 03:15:51

Fichier

7_PDFsam_COLSUB-D-19-01237R3_v...
Fichiers produits par l'(les) auteur(s)

Identifiants

Citation

Myolisi Ndumiso, Nela Buchtová, Lizex Husselmann, Gadija Mohamed, Ashwil Klein, et al.. Comparative whole corona fingerprinting and protein adsorption thermodynamics of PLGA and PCL nanoparticles in human serum. Colloids and Surfaces B: Biointerfaces, Elsevier, 2020, 188, pp.110816. ⟨10.1016/j.colsurfb.2020.110816⟩. ⟨hal-02778503⟩

Partager

Métriques

Consultations de la notice

33

Téléchargements de fichiers

82