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Article Dans Une Revue Journal of Materials Science: Materials in Medicine Année : 2017

Nanocomposite particles with improved microstructure for 3D culture systems and bone regeneration

Sergiu Cecoltan
  • Fonction : Auteur
Izabella-Cristina Stancu
  • Fonction : Auteur
Diana Dragusin
  • Fonction : Auteur
Andrada Serafim
  • Fonction : Auteur
Adriana Lungu
  • Fonction : Auteur
Cătălin Ţucureanu
  • Fonction : Auteur
Iuliana Caraş
  • Fonction : Auteur
Vlad Constantin Tofan
  • Fonction : Auteur
Aurora Sălăgeanu
  • Fonction : Auteur
Eugeniu Vasile
  • Fonction : Auteur
Cristin Coman
  • Fonction : Auteur
Mircea Istodorescu
  • Fonction : Auteur
Horia Iovu
  • Fonction : Auteur

Résumé

Nano-apatite and gelatin-alginate hydrogel microparticles have been prepared by a one-step synthesis combined with electrostatic bead generation, for the reconstruction of bone defects. Based on the analysis of bone composition, architecture and embryonic intramembranous ossification, a bio-inspired fabrication has been developed. Accordingly, the mineral phase has been in situ synthesized, calcifying the hydrogel matrix while the latter was crosslinked, finally generating microparticles that can assemble into a bone defect to ensure interconnected pores. Although nano-apatite-biopolymer composites have been widely investigated, microstructural optimization to provide improved distribution and stability of the mineral is rarely achieved. The optimization of the developed method progressively resulted in two types of formulations (15P and 7.5P), with 15 and 7.5 (wt%) phosphate content in the initial precursor. The osteolytic potential was investigated using differentiated macrophages. A commercially available calcium phosphate bone graft substitute (Eurocer 400) was incorporated into the hydrogel, and the obtained composites were in vitro tested for comparison. The cytocompatibility of the microparticles was studied with mouse osteoblast-like cell line MC3T3-E1. Results indicated the best in vitro performance have been obtained for the sample loaded with 7.5P. Preliminary evaluation of biocompatibility into a critical size (3 mm) defect in rabbits showed that 7.5P nanocomposite is associated with newly formed bone in the proximity of the microparticles, after 28 days.

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Dates et versions

hal-02869269 , version 1 (15-06-2020)

Identifiants

Citer

Sergiu Cecoltan, Izabella-Cristina Stancu, Diana Dragusin, Andrada Serafim, Adriana Lungu, et al.. Nanocomposite particles with improved microstructure for 3D culture systems and bone regeneration. Journal of Materials Science: Materials in Medicine, 2017, 28 (10), pp.153. ⟨10.1007/s10856-017-5966-8⟩. ⟨hal-02869269⟩

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