Nowadays, plants extract are more and more used in animal feed,as alternatives to antibiotic growth promoters. Some of them such as citrus extract had already shown beneficial effect on zootechnical performances and health. However, all citrus extracts can vary a lot in terms of concentration of active compounds. These variations may provoke efficacy differences. The study realised aimed to characterise a standardized natural citrus extract in order to identify the main components responsible for the effect. Then, the effect of this Standardized Natural Citrus Extract(SNCE) was evaluated through a meta-analysis in poultry.After freeze drying the citrus extract at 50 mg/mL (Nor-Spice® AB, Nor-Feed SAS, France), three HPLC analysis methods were performedusing HPLC-UV-DAD-DEDL (Shimadzu) and (HPLC UV-MSMS, Esquire 3000 Plus, Ion trap, Bruker) chromatographic systems. Analysis were performed using Hypersil RP-C18 column (4.6 mm, 250 mm, 5 μm) and data were analysed using DataAnalysis software. The main components of the product were identified using dereplication. In parallel, a meta-analysis was performed based on the results of seventeen trials realized between 1995 and 2018 with this Standardized Natural Citrus Extract. Each trial had 2 poultry groups, a control group (CTL group) fed with standard diet without supplementation and a SNCE group (SNCE group) supplemented with SNCE at dose between 250 and 400 ppm. Feed Conversion Ratio (FCR) and Average Daily Gain (ADG) were monitored in every trials. Trials were conducted worldwide. Statistical analysis were performed using Student test (T-test) and GraphPad Prism 7 (GraphPad software, USA). Spectral data from HPLC analysis allowed to identify approximately 30 secondary metabolites among carboxylic acids, phenolic acids, coumarin, flavones, flavanones and flavanols.Concerning the17 trials, 16 have shown a significant effect on FCR or ADG of broilers chickens (p<0.05, T-test). On average, SNCE supplementation on broilers significantly reduces the FCR by 2.3% and increases the ADG by 4.4% (p<0.05 Fisher Test)According to these data, Standardized Natural Citrus Extract supplementation seems to be an effective solution in order to improve zootechnical performances of broiler chickens and to offer a good alternative to antibacterial growth promoters