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Novel hybrid lipid nanocapsules loaded with a therapeutic monoclonal antibody –Bevacizumab– and Triamcinolone acetonide for combined therapy in neovascular ocular pathologies

Abstract : The aim of this study was to design and develop a novel hybrid formulation based on lipid nanocapsules containing bevacizumab (BVZ), an effective therapeutic antibody, on the surface and triamcinolone acetonide (TA) in the inner core (BVZ-TA-LNC) intended to improve ocular therapy. Hence, a phase inversion-insertion one step method was developed to drug loading and surface modification of lipid nanocapsules by post-insertion of a bifunctional polymer, followed by antibody oupling using “click” chemistry. The covalent bond and antibody capacity binding to its specific antigen were confirmed by thermal analysis and immunoassay, respectively. BVZTA- LNC presented nanometric size (102 nm), negative surface potential (−19 mV) and exhibiting 56% of TA in the lipid core. BVZ-TA-LNC tended to prevent the endothelial cell migration and significantly prevented the capillary formation induced by the vascular endothelium growth factor (VEGF). The novel hybrid system allowed the co-loading of two different therapeutic molecules and may be promising to mprove the therapy of eye disorders that occur with inflammation and/or neovascularization.
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https://hal.univ-angers.fr/hal-02926008
Contributeur : Laurent Lemaire <>
Soumis le : lundi 31 août 2020 - 11:45:51
Dernière modification le : jeudi 11 février 2021 - 15:40:27

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María Lina Formica, Samuel Legeay, Jérôme Bejaud, Guillermo Gabriel Montich, Gabriela Verónica Ullio Gamboa, et al.. Novel hybrid lipid nanocapsules loaded with a therapeutic monoclonal antibody –Bevacizumab– and Triamcinolone acetonide for combined therapy in neovascular ocular pathologies. Materials Science and Engineering: C, Elsevier, 2021, 119, pp.111398. ⟨10.1016/j.msec.2020.111398⟩. ⟨hal-02926008⟩

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