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Reliability of real-time RT-PCR tests to detect SARS-Cov-2: A literature review

Abstract : In the face of the COVID-19 (Coronavirus Disease 2019) pandemic, the World Health Organization (WHO) has urged countries to test the population more widely. Clinical laboratories have been confronted with a huge demand for testing and have had to make urgent preparations for staff training, to establish new analytical processes, reorganize the workspace, and stock up on specific equipment and diagnostic test kits. The reliability of SARS-Cov-2 test results is of critical importance, given the impact it has on patient care and the management of the health crisis. A review of the literature available for the period leading up to and including June 2020 on the reliability of SARS-Cov-2 (Severe Acute Respiratory Syndrome Coronavirus) detection methods using real-time RT PCR (Reverse Transcription-Polymerase Chain Reaction) brings together the primary factors teams of scientists claim or demonstrate to affect the reliability of results. A description is given of the RT-PCR testing method, followed by a presentation of the characteristics and validation techniques used. A summary of data from the literature on the reliability of tests and commercial kits for SARS-Cov-2 detection, including current uncertainties with regard to the molecular targets selected and genetic diversity of SARS-Cov-2 is provided. The limitations and perspectives are then discussed in detail in the light of the bibliographic data available. Many questions have been asked that still remain unanswered. The lack of knowledge about this novel virus, which appeared at the end of 2019, has a significant impact on the technical capacity to develop reliable, rapid and practical tools for its detection.
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https://hal.univ-angers.fr/hal-03024214
Contributeur : Abdérafi Charki <>
Soumis le : mercredi 25 novembre 2020 - 17:19:03
Dernière modification le : lundi 4 janvier 2021 - 11:52:32

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Clément Bezier, Géraldine Anthoine, Abdérafi Charki. Reliability of real-time RT-PCR tests to detect SARS-Cov-2: A literature review. International Journal of Metrology and Quality Engineering, EDP sciences, 2020, ⟨10.1051/ijmqe/2020014⟩. ⟨hal-03024214⟩

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