Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Carbapenem Resistance and Acinetobacter baumannii in Senegal: The Paradigm of a Common Phenomenon in Natural Reservoirs

Abstract :

Incidence of carbapenem-resistant Acinetobacter baumannii is rising in several parts of the world. In Africa, data concerning this species and its resistance to carbapenems are limited. The objective of the present study was to identify the presence of A. baumannii carbapenem-resistant encoding genes in natural reservoirs in Senegal, where antibiotic pressure is believed to be low. From October 2010 to January 2011, 354 human head lice, 717 human fecal samples and 118 animal fecal samples were screened for the presence of A. baumannii by real time PCR targeting blaOXA51-like gene. For all samples positive for A. baumannii, the carbapenemase-hydrolysing oxacillinases blaOXA23-like and blaOXA24-like were searched for and sequenced, and the isolates harbouring an oxacillinase were genotyped using PCR amplification and sequencing of recA gene. The presence of A. baumannii was detected in 4.0% of the head lice, in 5.4% of the human stool samples and in 5.1% of the animal stool samples tested. No blaOXA24 gene was detected but six fecal samples and three lice were positive for blaOXA23-like gene. The blaOXA23-like gene isolated in lice was likely a new oxacillinase sequence. Finally, the A. baumannii detected in stools were all of recA genotype 3 and those detected in lice, of recA genotype 4. This study shows for the first time a reservoir of blaOXA23-like-positive gene in human head lice and stool samples in Senegal.

Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03134640
Contributeur : Okina Université d'Angers <>
Soumis le : lundi 8 février 2021 - 14:27:11
Dernière modification le : mercredi 3 mars 2021 - 18:34:02
Archivage à long terme le : : dimanche 9 mai 2021 - 19:26:44

Fichier

journal.pone_.0039495.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Marie Kempf, Jean-Marc Rolain, Georges Diatta, Saïd Azza, Bissoum Samb, et al.. Carbapenem Resistance and Acinetobacter baumannii in Senegal: The Paradigm of a Common Phenomenon in Natural Reservoirs. PLoS ONE, Public Library of Science, 2012, 7 (6), Non spécifié. ⟨10.1371/journal.pone.0039495⟩. ⟨hal-03134640⟩

Partager

Métriques

Consultations de la notice

65

Téléchargements de fichiers

70