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Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis

Abstract :

Mycobacterium ulcerans is the causative agent of Buruli ulcer, a rapidly emerging human disease in which mycolactone, a cytotoxic and immunosuppressive macrocyclic polyketide, is responsible for massive skin destruction. The genome sequencing of M. ulcerans has recently been accomplished (http://genolist.pasteur.fr/BuruList/) enabling the first proteome study of this important human pathogen. Here, we present a comprehensive proteome analysis of different subcellular fractions and culture supernatant of in vitro grown M. ulcerans. By a combination of gel-based and gel-free techniques for protein and peptide separation with subsequent analysis by MS, we identified 1074 different proteins, corresponding to 25% of the protein-coding DNA sequence. Interestingly, new information was obtained about central metabolism and lipid biosynthesis, and as many as 192 conserved hypothetical proteins were found. Comparative analysis of the wild-type strain and an isogenic mycolactone-deficient mutant, by 2-DE and iTRAQ labeling of the cytoplasmic fraction, revealed differences in the expression profiles of proteins involved in lipid metabolism and information pathways, as well as stress responses.

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https://hal.univ-angers.fr/hal-03134754
Contributeur : Okina Université d'Angers <>
Soumis le : lundi 8 février 2021 - 14:56:26
Dernière modification le : vendredi 17 septembre 2021 - 14:50:09

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Petra Tafelmeyer, Christine Laurent, Pascal Lenormand, Jean-Claude Rousselle, Laurent Marsollier, et al.. Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis. Proteomics, Wiley-VCH Verlag, 2008, 8 (15), pp.3124 - 3138. ⟨10.1002/pmic.200701018⟩. ⟨hal-03134754⟩

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