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Article dans une revue

Molecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata

Abstract :

Resistance to 5-fluorocytosine (5-FC) has been poorly investigated in the yeast Candida glabrata. This study was conducted on laboratory mutants obtained by exposure of a wild-type isolate to 5-FC. Based on their susceptibility to 5-fluorouracil (5-FU), two of these mutants were selected for further analysis of the molecular mechanisms of 5-FC resistance. One mutant, resistant to both compounds, exhibited a missense mutation in the gene coding the cytosine deaminase and a decrease in the expression level of the gene coding the uridine monophosphate pyrophosphorylase. The other mutant that showed a reduced susceptibility to 5-FC and 5-FU exhibited an overexpression of the genes coding the thymidylate synthase and a cytosine permease, associated with a missense mutation in the last gene. Thus, beside mutations in the FUR1 gene which represent the most common cause of resistance to 5-FC, other mechanisms may also occur in C. glabrata.

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https://hal.univ-angers.fr/hal-03134774
Contributeur : Okina Université d'Angers <>
Soumis le : lundi 8 février 2021 - 14:57:02
Dernière modification le : vendredi 12 février 2021 - 18:27:17

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Patrick Vandeputte, Laurent Pineau, Gérald Larcher, Thierry Noel, Daniel Brèthes, et al.. Molecular Mechanisms of Resistance to 5-Fluorocytosine in Laboratory Mutants of Candida glabrata. Mycopathologia, 2011, 171 (1), pp.11 - 21. ⟨10.1007/s11046-010-9342-1⟩. ⟨hal-03134774⟩

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