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Communication dans un congrès

Sequential variations of Rasamsonia spp. isolate genotypes in cystic fibrosis patients

Abstract :

Rasamsonia species have been recently described as emergent pathogens in human and animals. Recent multilocus phylogenetic studies have shown that the genus Rasamsonia (R.) includes at least four phylogenetically related species forming the R. argillacea complex, R. argillacea sensu stricto, R. piperina, R. eburnea and R. aegroticola, which have been mostly reported in the clinical context of chronic granulomatous disease (CGD) and cystic fibrosis (CF). Differences in sensitivity to antifungal therapies which have been noted within the genus Rasamsonia underline the importance of early identification of clinical isolates for optimal treatment during the early stages of infection. In this context, the aim of this work was to obtain genotypic characterization of clinical Rasamsonia spp. isolates from 26 CF patients and one acutely infected CGD patient using PCR amplification of repetitive DNA sequences (rep-PCR) and to compare species distributions
determined by β tubulin sequencing with rep-PCR genotypes/clusters.

One hundred and ten isolates were collected sequentially or simultaneously from 01/09/1998 to 08/2012 from sputum samples of 26 CF patients. One CGD patient (4 isolates) was studied in parallel. Two references strains (CBS #408.73 (R. piperina) and #432.62 (R. cylindrospora) were obtained from CBS. After extraction, PCR amplifications of repetitive DNA sequences were realized for each sample using the DiversiLab Fungal kit (bioMérieux). Amplicons were separated by capillary electrophoresis in an Agilent 2100 Bioanalyzer (Massy, France). Single electrophoretic profiles obtained for each isolate were compared using the DiversiLab Healthcare software (version 3.41).

As previously reported in CF patients, beta-tubulin gene sequencing of 110 isolates identified more frequently R. aegroticola (72 isolates from 19 CF patients) than R. argillacea stricto sensu (30 isolates from 8 CF patients), and R. piperina (8 isolates from 5 CF patients), with 15, 6, and 7 distinct profiles, respectively. From the CGD patient, all isolates were identified as R. argillacea stricto sensu. Without exception, species identification according to rep-PCR genotypes was entirely consistent with beta-tubulin sequencing results.

Present data provide the first description of sequential Rasamsonia spp. variations with time in CF patients. Rasamsonia argillacea sensu stricto isolates were found persistent for years in all sequentially sampled CF patients except 1 who presented 4 different successive genotypes. In contrast to R. argillacea sensu
stricto
, sequential detection of R. aegroticola and R. piperina revealed frequent successive changes in isolate genotypes in most CF patients.

Type de document :
Communication dans un congrès
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https://hal.univ-angers.fr/hal-03136191
Contributeur : Okina Université d'Angers <>
Soumis le : mardi 9 février 2021 - 15:21:06
Dernière modification le : lundi 17 mai 2021 - 14:52:24

Identifiants

  • HAL Id : hal-03136191, version 1
  • OKINA : ua11250

Citation

Olivier Matray, A Mouhajir, Sandrine Giraud, Gilles Gargala, Rachid Zouhair, et al.. Sequential variations of Rasamsonia spp. isolate genotypes in cystic fibrosis patients. Third Meeting of the ECMM/ISHAM Working group Fungal respiratory infections in Cystic Fibrosis (Fri-CF), 2014, Angers, France. ⟨hal-03136191⟩

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