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Article dans une revue

SiRNA LNCs - A novel platform of lipid nanocapsules for systemic siRNA administration

Abstract :

Several siRNA (small interfering RNA) therapeutics are undergoing clinical trials for cancer, respiratory diseases or macular degeneration, but most are administrated locally. In order to overcome the different barriers to attain an efficient siRNA action after systemic administration, nanocarriers able to carry and protect siRNA are awaited. With this aim, we developed a new platform of siRNA lipid nanocapsules (LNCs) using different cationic lipids, combining the properties of LNCs (siRNA protection and targeting) and lipoplexes (efficient siRNA delivery into the cell). The formulation was revealed to contain different compartments. A siRNA quantification method based on UV spectroscopy was developed to locate and quantify siRNA in each compartment. All in all, these novel siRNA LNCs presented sizes of about 55 nm with a neutral surface charge and siRNA encapsulation efficiencies up to 65% representing appropriate characteristics for systemic administration.

Type de document :
Article dans une revue
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Contributeur : Okina Université d'Angers <>
Soumis le : mercredi 10 mars 2021 - 16:30:57
Dernière modification le : vendredi 12 mars 2021 - 13:33:34
Archivage à long terme le : : vendredi 11 juin 2021 - 19:12:22


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Stephanie David, Pauline Resnier, A. Guillot, Bruno Pitard, Jean-Pierre Benoit, et al.. SiRNA LNCs - A novel platform of lipid nanocapsules for systemic siRNA administration. European Journal of Pharmaceutics and Biopharmaceutics, Elsevier, 2012, 81 (2), pp.448-42. ⟨10.1016/j.ejpb.2012.02.010⟩. ⟨hal-03165467⟩



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