Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Action of Humicola lanuginosa lipase on mixed monomolecular films of tricaprylin and polyethylene glycol stearate

Abstract :

The hydrolysis catalyzed by Humicola lanuginosa lipase (HLL) of pure tricaprylin (TC) or stearate of polyethylene glycol 1500 (PEG-St) as well as their mixtures spread as monomolecular films were studied. The catalytic transformation of the two substrates TC or PEG-St into their respective reaction products was detected by measuring simultaneously the decrease in the film area and the surface potential using the "zero order" trough at constant surface pressure. A kinetic model describing the enzymatic hydrolysis was developed. The surface concentrations of the two substrates and their respective reaction products as well as the values of the global kinetic constants of hydrolysis were determined. The experimentally obtained global kinetic constants of the catalytic action of HLL against TC and PEG-St present in mixed monolayers of TC/PEG-St are approximately the same as in the case of pure monolayers. These obtained results give some indications that the activity of enzyme is not significantly affected by the different molecular environments in the mixed monolayers.

Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03171788
Contributeur : Okina Université d'Angers <>
Soumis le : mercredi 17 mars 2021 - 11:12:26
Dernière modification le : jeudi 18 mars 2021 - 03:20:27

Identifiants

  • HAL Id : hal-03171788, version 1
  • OKINA : ua3771

Collections

Citation

Tzvetanka Ivanova, Kristina Mircheva, G. Dobreva, Ivan Panaiotov, J. Proust, et al.. Action of Humicola lanuginosa lipase on mixed monomolecular films of tricaprylin and polyethylene glycol stearate. Colloids and Surfaces B: Biointerfaces, Elsevier, 2008, 63 (1), pp.91-100. ⟨hal-03171788⟩

Partager

Métriques

Consultations de la notice

10