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Interactions between poly(ethylene glycol) and protein in dichloromethane/water emulsions. 2. Conditions required to obtain spontaneous emulsification allowing the formation of bioresorbable poly(D,L lactic acid) microparticles

Abstract :

From microscopic observations, it was established that an oil-in-water emulsion with droplets of a size in the micrometer range can spontaneously form at room temperature without additional external stirring as soon as a solvent that is only partly miscible to water-like dichloromethane (DCM) is put in contact with an aqueous mixture of polyethylene glycol (PEG) and a protein. Experimental results show that emulsification only occurs if the system simultaneously includes PEG with middle chain, an organic solvent partly miscible to water and for which PEG affinity is sufficiently high, and a protein. From adsorption kinetics, it appears that this spontaneous emulsification process is related to the rapid diffusion of DCM towards water through the formation of interfacial turbulences, once the accumulation of PEG close to the DCM/water interface occurs. The oil droplets formed would be then stabilized by adsorbed protein molecules. Since the presence of polylactic acid in the organic phase did not prevent the emulsion formation, we studied the feasibility of formulating microparticles using this polymer. From results, it appears that microcapsules with a polymeric shell, with a homogeneous size of about 50 microm and able to encapsulate a model hydrophobic drug, such as amiodarone, can be obtained by using this spontaneous emulsification method.

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Article dans une revue
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https://hal.univ-angers.fr/hal-03171800
Contributeur : Okina Université d'Angers <>
Soumis le : mercredi 17 mars 2021 - 11:12:48
Dernière modification le : jeudi 18 mars 2021 - 03:20:11

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  • HAL Id : hal-03171800, version 1
  • OKINA : ua3740

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Citation

A. Malzert-Freon, K. Schonhammer, Jean-Pierre Benoît, Frank Boury. Interactions between poly(ethylene glycol) and protein in dichloromethane/water emulsions. 2. Conditions required to obtain spontaneous emulsification allowing the formation of bioresorbable poly(D,L lactic acid) microparticles. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, 2009, 73 (1), pp.66-73. ⟨hal-03171800⟩

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