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Human glioma cell culture: Two FCS-free media could be recommended for clinical use in immunotherapy

Abstract : Immunotherapy, particularly active vaccination, may be developed as an effective and safe treatment modality for malignant gliomas, which continue to have a poor prognosis, despite advances in surgical techniques and adjuvant chemotherapy and radiotherapy. Since no glioma-specific tumor-associated antigens (TAAs) have been discovered, autologous tumor cells or well-established glioma cell lines could be used in future vaccination protocols to induce antitumour immunity against unknown TAAs. One obstacle for clinical use of these tumour cell vaccines is related to foetal calf serum (FCS). Efforts are currently being directed toward developing FCS-free media and serum-free alternatives to culture these cell vaccines. In this study, a medium containing human serum and one serum-free medium (UltraCulture), supplemented or not with epidermal growth factor, were tested on morphology, survival, DNA content and TAA expression of human glioma cell lines and glioma biopsy primary cultures. Their effects were compared on FCS-containing medium. Results show that, whatever the medium used, no significant variations in morphology and survival were observed. Furthermore, human serum-containing medium or UltraCulture preserved at early passage cultures the cell population of interest present in the biopsies before culture. In addition, the expression profile of eight TAAs was similar between these media. These data indicate that human serum-containing medium and UltraCulture serum-free medium could be promising candidates to produce tumour-cell vaccines.
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Soumis le : mercredi 17 mars 2021 - 11:13:06
Dernière modification le : lundi 25 octobre 2021 - 15:10:03

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Anne Clavreul, Isabelle Jean, Laurence Preisser, Agnès Chassevent, A. Sapin, et al.. Human glioma cell culture: Two FCS-free media could be recommended for clinical use in immunotherapy. In Vitro Cellular & Developmental Biology - Animal, Springer, 2009, 45 (9), pp.500-511. ⟨10.1007/s11626-009-9215-4⟩. ⟨hal-03171815⟩



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