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Article dans une revue

The encapsulation of DNA molecules within biomimetic lipid nanocapsules

Abstract :

Most of DNA synthetic complexes result from the self-assembly of DNA molecules with cationic lipids or polymers in an aqueous controlled medium. However, injection of such self-assembled complexes in medium like blood that differ from that of their formulation leads to strong instability. Therefore, DNA vectors that have physico-chemical properties and structural organisation that will not be sensitive to a completely different medium in terms of ionic and protein composition are actively sought. To this end, the goal here was to discover and optimize a nanostructured system where DNA molecules would be encapsulated in nanocapsules consisting in an oily core and a shell covered by PEG stretches obtained through a nanoemulsion process in the absence of organic solvent. This encapsulation form of DNA molecules would prevent interactions with external hostile biological fluid. The results show the entrapment of lipoplexes into lipid nanocapsules, leading to the formation of neutral 110 nm-DNA nanocapsules. They were weakly removed by the immune system, displaying an increased blood half-life, and improved carcinoma cell transfection, in comparison to the parent lipoplexes. Our results demonstrate that the fabrication of nanocapsules encapsulating hydrophilic DNA in an oily core that meet criteria for blood injection is possible.

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Article dans une revue
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https://hal.univ-angers.fr/hal-03171863
Contributeur : Okina Université d'Angers <>
Soumis le : mercredi 17 mars 2021 - 11:25:08
Dernière modification le : jeudi 18 mars 2021 - 03:20:20

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Arnaud Vonarbourg, Catherine Passirani-Malleret, L. Desigaux, Emilie Allard-Vannier, Patrick Saulnier, et al.. The encapsulation of DNA molecules within biomimetic lipid nanocapsules. Biomaterials, Elsevier, 2009, 30 (18), pp.3197-204. ⟨10.1016/j.biomaterials.2009.03.009⟩. ⟨hal-03171863⟩

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