Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Anti-epidermal growth factor receptor siRNA carried by chitosan-transacylated lipid nanocapsules increases sensitivity of glioblastoma cells to temozolomide

Abstract : Epidermal growth factor receptor (EGFR) is a crucial protein that plays an important role in the maintenance and development of glioblastomas. The silencing or knockdown of EGFR is possible by administering a small interfering ribonucleic acid (siRNA). Lipid nanocapsules (LNCs) covered by chitosan were developed in our laboratory by a transacylation process. The resulting nanocapsules have a positive zeta potential that enables electrostatic interactions with the negatively-charged siRNA. Prior to transfection, the cytotoxicity of the nanocapsules by (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) test was performed on the U87MG cell line to determine non-toxic levels of the LNCs to avoid cell mortality. Treatment of the U87MG cells with the chitosan-transacylated LNCs/anti-EGFR siRNA complex resulted in a reduction of EGFR expression by 51.95%±6.03% (P≤0.05) after 96 hours of incubation. It also increased the cellular sensitivity to temozolomide in comparison to untreated cells with siRNA. The largest increase in mortality was 62.55%±3.55% (P<0.05). This successful knockdown provides proof for the concept of surface grafting of siRNA onto LNCs to modify cell sensitivity to temozolomide. The method could be implemented in future clinical models regarding the experimental treatment of glioblastoma cancer..
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03179493
Contributeur : Okina Université d'Angers <>
Soumis le : mercredi 24 mars 2021 - 12:22:30
Dernière modification le : vendredi 26 mars 2021 - 03:03:45

Fichier

messaoudi_2014_anti_epidermal_...
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité - Pas d'utilisation commerciale 4.0 International License

Identifiants

Collections

Citation

Khaled Messaoudi, Patrick Saulnier, Kim Boesen, Jean-Pierre Benoit, Frédéric Lagarce. Anti-epidermal growth factor receptor siRNA carried by chitosan-transacylated lipid nanocapsules increases sensitivity of glioblastoma cells to temozolomide. International Journal of Nanomedicine, Dove Medical Press, 2014, 9, pp.1479-90. ⟨10.2147/IJN.S59134⟩. ⟨hal-03179493⟩

Partager

Métriques

Consultations de la notice

40

Téléchargements de fichiers

26