Phenolic derivatives such as quinones, acid-phenols and flavonoids were successfully isolated from a n-butanolic fraction of Senecio giganteus Desf. (Asteraceae) flowers, namely jacaranone (1), 3a-hydroxy-3,3a,7,7a-tetrahydrobenzofuran-2,6-dione (2), chlorogenic acid (3), hyperoside (4), quercetin 3-O-β-D-robinobioside (5), isorhamnetin-3-O-β-D-glucuronide (6), quercetin-3-O-β-D-glucuronide (7), and isorhamnetin-3-O-β-D-glucuronide-6″-methyl ester (8). These compounds were purified through either classical polyamide filtration followed by fractionation on Si gel, or through fast centrifugal partition chromatography (FCPC). Using FCPC, the major compounds could be readily isolated from the crude n-butanolic fraction. Compounds 1-8 were identified by means of spectroscopic and spectrometric analysis (UV, ¹H, ¹³C and 2D NMR, and MS). This work described for the first time the phytochemical composition of this endemic Algerian plant.