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Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts

Abstract :

Among nineteen evaluated Clusiaceous species, one stem bark CH2Cl2 crude extract was selected based on a significant inhibition of acetylcholinesterase (AChE) using the micro-dilution Ellman's method [1]. A normal phase HPLC profiling with micro-fractionation of this extract provided discrete fractions every 20 seconds. In order to obtain a comprehensive profiling of AChE activity all microfractions were tested [2] in dilution assay (Ellman) as well as by bioautography (the Fast Blue B salt method). Furthermore the potency of inhibition was evaluated both by keeping the genuine concentration within the extract and after normalisation to a standard concentration level. From the active fractions five pure compounds were isolated and identified. The different methods of sample preparation and biological evaluation associated with normal-phase micro-fractionation of plant extracts are critically discussed.

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Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : jeudi 3 juin 2021 - 10:17:14
Dernière modification le : jeudi 2 décembre 2021 - 14:26:05



Anne Landreau, Samuel Bertrand, Claudia Simoes-Pires, Laurence Marcourt, T-D Bach, et al.. Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts. International Congress on Natural Products Research on Global Change, Natural Products and Human Health/8th Joint Meeting of AFERP, ASP, GA, PSE and SIF, 2012, New-York, United States. Planta Medica, 78 (11), p. 1262, 2012, ⟨10.1055/s-0032-1321222⟩. ⟨hal-03247507⟩



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