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Article dans une revue

Dynamic Nature of the Ligustilide Complex

Abstract :

Monomeric phthalides such as Z-ligustilide (1) and Z-butylidenephthalide (2) are major constituents of medicinal plants of the Apiaceae family. While 1 has been associated with a variety of observed biological effects, it is also known for its instability and rapid chemical degradation. For the purpose of isolating pure 1 and 2, a gentle and rapid two-step countercurrent isolation procedure was developed. From a supercritical CO2 fluid extract ofAngelica sinensis roots, the phthalides were isolated with high GC-MS purities of 99.4% for 1and 98.9% for 2 and consistently lower qHNMR purities of 98.1% and 96.4%, respectively. Taking advantage of molarity-based qHNMR methodology, a time-resolved study of the dynamic changes and residual complexity of pure 1 was conducted. GC-MS and (qH)NMR analysis of artificially degraded 1 provided evidence for the phthalide degradation pathways and optimized storing conditions. Parallel qHNMR analysis led to the recognition of variations in time- and process-dependent sample purity and has impact on the overall assessment of time-dependent changes in complex natural products systems. The study underscores the importance of independent quantitative monitoring as a prerequisite for the biological evaluation of labile natural products such as monomeric phthalides.

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Article dans une revue
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https://hal.univ-angers.fr/hal-03247824
Contributeur : Okina Université d'Angers <>
Soumis le : jeudi 3 juin 2021 - 11:41:17
Dernière modification le : vendredi 4 juin 2021 - 03:10:02

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Andreas Schinkovitz, Samuel Pro, Matthew Main, Shaonong Chen, Birgit Jaki, et al.. Dynamic Nature of the Ligustilide Complex. Journal of Natural Products, American Chemical Society, 2008, 71 (9), pp.1604-11. ⟨10.1021/np800137n⟩. ⟨hal-03247824⟩

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