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In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate

Abstract :

Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg-Gly-Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a bisphosphonate used as an anchor system and chemically adsorbed on polished titanium discs. Two different concentrations, 10−10 mol/L (RGD 10−10) and 10−4 mol/L (RGD 10−4) were compared to non coated discs (RGD 0). Adhesion, spreading, and mineralization of osteoblast-like cells (Saos-2) were assessed. Mineralization kinetic was done at 3, 6, 10, 14, and 18 days of culture; the extent of mineral deposits was quantified by image analysis. Histogram repartitions of nuclear area, characterizing cell spreading, showed a shift to higher values in cells cultured on RGD coated titanium disks. Mineralization started at day 3 in the three groups, but had a faster development in the RGD 10−10 group from day 6 to day 18 compared to RGD 0 and RGD 10−4. At day 18, the percentage of mineralized area was significantly higher for RGD 10−10 compared to RGD 0 (p < 0.05). In the present study, this new method was found suitable to anchor RGD containing species on titanium: this favored adhesion and spreading of osteoblast-like cells and mineralization compared to noncoated titanium.

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https://hal.univ-angers.fr/hal-03261974
Contributeur : Okina Université d'Angers <>
Soumis le : mercredi 16 juin 2021 - 11:35:22
Dernière modification le : jeudi 17 juin 2021 - 03:43:33

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Johanne Beuvelot, David Portet, Grégory Lecollinet, Marie-Françoise Moreau, Michel-Félix Baslé, et al.. In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate. Journal of Biomedical Materials Reserach Part B Applied Biomaterials, Wiley, 2009, 90B (2), pp.873 - 881. ⟨10.1002/jbm.b.31357⟩. ⟨hal-03261974⟩

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