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Article dans une revue

PPARα is essential for microparticle-induced differentiation of mouse bone marrow-derived endothelial progenitor cells and angiogenesis

Abstract :


Bone marrow-derived endothelial progenitor cells (EPCs) are critical for neovascularization. We hypothesized that microparticles (MPs), small fragments generated from the plasma membrane, can activate angiogenic programming of EPCs.

Methodology/Principal Findings

We studied the effects of MPs obtained from wild type (MPsPPARα+/+) and knock-out (MPsPPARα−/−) mice on EPC differentiation and angiogenesis. Bone marrow-derived cells were isolated from WT or KO mice and were cultured in the presence of MPsPPARα+/+ or MPsPPARα−/− obtained from blood of mice. Only MPsPPARα+/+ harboring PPARα significantly increased EPC, but not monocytic, differentiation. Bone marrow-derived cells treated with MPsPPARα+/+ displayed increased expression of pro-angiogenic genes and increased in vivo angiogenesis. MPsPPARα+/+ increased capillary-like tube formation of endothelial cells that was associated with enhanced expressions of endothelial cell-specific markers. Finally, the effects of MPsPPARα+/+ were mediated by NF-κB-dependent mechanisms.


Our results underscore the obligatory role of PPARα carried by MPs for EPC differentiation and angiogenesis. PPARα-NF-κB-Akt pathways may play a pivotal stimulatory role for neovascularization, which may, at least in part, be mediated by bone marrow-derived EPCs. Improvement of EPC differentiation may represent a useful strategy during reparative neovascularization.

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Article dans une revue
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Soumis le : jeudi 1 juillet 2021 - 09:23:11
Dernière modification le : mercredi 19 janvier 2022 - 16:22:03
Archivage à long terme le : : samedi 2 octobre 2021 - 18:27:59


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Tarek Benameur, Simon Tual-Chalot, Ramaroson Andriantsitohaina, Maria Carmen Martinez. PPARα is essential for microparticle-induced differentiation of mouse bone marrow-derived endothelial progenitor cells and angiogenesis. PLoS ONE, Public Library of Science, 2010, 5 (8), pp.e12392. ⟨10.1371/journal.pone.0012392⟩. ⟨hal-03275381⟩



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