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Article dans une revue

Attenuation of allergic airways inflammation by an extract of Hymenocardia acida

Abstract :

Tracheal hyperresponsiveness, airway mucus production and bronchoalveolar inflammation are the major components of asthma. Here, we aim to investigate the role in the control of asthma of a bioactive plant extracted from Hymenocardia acida in a physiological and pathophysiological model. The effect of H. acida crude extract (HACE) on total cellular components of bronchoalveolar (BAL) fluids was performed on ovalbumin (OVA) and lipopolysaccaride (LPS)-challenged Swiss mice for induction of allergic asthma and airways inflammation, respectively. Mice were pretreated with 0.9% sodium chloride (NaCl), HACE (oral doses at 100 mg/kg/body weight) for a week and then by intranasal instillation with OVA (0.5 mg/ml) + aluminium hydroxyde (20 mg/ml), during three days after intraperitoneally sensitization or with LPS (0.4 mg/ml) for a day (OVA or LPS + HACE). The BAL cells were collected in a mixed solution (0.9% NaCl and 2.6 mm Ethylenediaminetetraacetic acid EDTA) one day after the last challenge and total cells were numbered in a Neubauer chamber. The HACE: (i) significantly inhibited the airways inflammation induced by a single intranasal instillation of LPS or allergic asthma on mice challenged with 3 consecutive days intranasal instillation of OVA in comparison to control mice only instilled with 0.9% sterile. NaCl : (ii) significantly impaired the increased levels of total cells in OVA and LPS-treated mice, without changing the basal cellularity after NaCl or HACE treatment; (iii) and significantly inhibitshydroxyl radicalsandsuperoxideanions production. Taken together, these results suggest that HACE exposure induces a marked reduction of cellular component in the BAL fluid, which is only partially lymphocytes dependent.

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Article dans une revue
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Soumis le : vendredi 2 juillet 2021 - 11:43:44
Dernière modification le : mercredi 19 janvier 2022 - 16:22:03


  • HAL Id : hal-03276619, version 1
  • OKINA : ua10512



Fatou Sar, Mamadou Sarr, Mama Diallo, Saliou Ngom, Lamine Gueye, et al.. Attenuation of allergic airways inflammation by an extract of Hymenocardia acida. Journal of Physiology and Pathophysiology, 2014, 5 (3), pp.15-24. ⟨hal-03276619⟩



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