Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Lower Funneling Pathways in Scedosporium Species

Abstract : Lignin, a natural polyaromatic macromolecule, represents an essential component of the lignocellulose biomass. Due to its complexity, the natural degradation of this molecule by microorganisms still remains largely misunderstood. Extracellular oxidative degradation is followed by intracellular metabolic degradation of conserved aromatic intermediate compounds (protocatechuate, catechol, hydroxyquinol, and gentisic acid) that are used as carbon and energy sources. The lower funneling pathways are characterized by the opening of the aromatic ring of these molecules through dioxygenases, leading to degradation products that finally enter into the tricarboxylic acid (TCA) cycle. In order to better understand the adaptation mechanisms of Scedosporium species to their environment, these specific catabolism pathways were studied. Genes encoding ring-cleaving dioxygenases were identified in Scedosporium genomes by sequence homology, and a bioinformatic analysis of the organization of the corresponding gene clusters was performed. In addition, these predictions were confirmed by evaluation of the expression level of the genes of the gentisic acid cluster. When the fungus was cultivated in the presence of lignin or gentisic acid as sole carbon source, experiments revealed that the genes of the gentisic acid cluster were markedly overexpressed in the two Scedosporium species analyzed ( Scedosporium apiospermum and Scedosporium aurantiacum ). Only the gene encoding a membrane transporter was not overexpressed in the gentisic acid-containing medium. Together, these data suggest the involvement of the lower funneling pathways in Scedosporium adaptation to their environment.
Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Nathalie Clement Connectez-vous pour contacter le contributeur
Soumis le : mardi 20 juillet 2021 - 14:19:10
Dernière modification le : mercredi 19 janvier 2022 - 16:34:02

Lien texte intégral




Wilfried Poirier, Kevin Ravenel, Jean-Philippe Bouchara, Sandrine Giraud. Lower Funneling Pathways in Scedosporium Species. Frontiers in Microbiology, Frontiers Media, 2021, 12, ⟨10.3389/fmicb.2021.630753⟩. ⟨hal-03292404⟩



Consultations de la notice