Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Siderophore Production by Pathogenic Mucorales and Uptake of Deferoxamine B

Abstract :

Clinical reports have established that mucormycosis, mainly caused by Rhizopus spp., frequently occurs in patients treated with deferoxamine B (DFO, Desferal®) which is misappropriated by these fungi. Siderophore production by twenty mucoralean isolates was therefore investigated using a commercial iron-depleted culture medium. Siderophore production was detected for most of the isolates. Our experiments confirmed that feroxamine B (iron chelate of DFO) promoted in vitro growth of Rhizopus arrhizus. Electrophoretic analysis of somatic extracts revealed iron-regulated proteins of 60 and 32 kDa which were lacking in iron-depleted culture conditions. Using a fluorescent derivative of deferoxamine B, we showed by fluorescence microscopy the entry of the siderophore within the fungal cells, thus suggesting a shuttle mechanism encompassing the uptake of the entire siderophore-ion complex into the cell. This useful tool renders possible a better understanding of iron metabolism in Mucorales which could lead to the development of new diagnostic method or new antifungal therapy using siderophores as imaging contrast agents or active drug vectors.

Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03332244
Contributeur : Okina Université d'Angers <>
Soumis le : jeudi 2 septembre 2021 - 15:38:02
Dernière modification le : mercredi 8 septembre 2021 - 03:30:39

Fichier

art3a10.10072fs11046-013-9693-...
Accord explicite pour ce dépôt

Identifiants

Citation

Gérald Larcher, Marylène Dias, Bienvenue Razafimandimby, Danielle Bomal, Jean-Philippe Bouchara. Siderophore Production by Pathogenic Mucorales and Uptake of Deferoxamine B. Mycopathologia, 2013, 176 (5-6), pp.319-328. ⟨10.1007/s11046-013-9693-5⟩. ⟨hal-03332244⟩

Partager

Métriques

Consultations de la notice

7

Téléchargements de fichiers

3