Transcriptomic Analysis Highlights Time-specific Embryonic Adaptation of Mice to the Lack of PrP - Université d'Angers Accéder directement au contenu
Article Dans Une Revue Prion Année : 2010

Transcriptomic Analysis Highlights Time-specific Embryonic Adaptation of Mice to the Lack of PrP

Manal Khalife
  • Fonction : Auteur
Rachel Young
  • Fonction : Auteur
Sead Chadi
  • Fonction : Auteur
Sandrine Le Guillou
Lucas Lefevre
  • Fonction : Auteur
Gaëlle Tilly
  • Fonction : Auteur
Frédérique Bitton
Marie-Laure Martin-Magniette
Ludivine Soubigou-Taconnat
  • Fonction : Auteur
Sandrine Balzergue
  • Fonction : Auteur
Marthe Vilotte
  • Fonction : Auteur
Bruno Passet
Vincent Béringue
Fabienne Le Provost
Hubert Laude
  • Fonction : Auteur
Jean-Luc Vilotte

Résumé

The physiological function of the PrP remains largely elusive. Its invalidation does not affect mouse survival and induces subtle phenotypes. To potentially assess this conundrum, we first comparatively analyzed the adult brain transcriptome of wild-type mice with that of transgenic mice invalidated at this locus either at the zygotic (Zürich PrP0/0 mice) or adult stages (NFH-Cre-Lox mice). Only subtle differences could be evidenced in the adult brains following microarray and QPCR analyses. When performed at an early adult stage, neuronal Prnp disruption appeared to sequentially induce an oxidative stress response and a nervous system remodeling, but it involved a limited number of only slightly modified genes. In sharp contrast, analysis at early embryonic stages, 7.5 and 8.5 dpc, just after the suspected normal time set of the Prnp locus activation, led to a transient perturbation of the transcriptome involving a larger number of genes and pointing to potential pathways related to the PrP physiological function. Overall, our data suggests an early adaptation of the mouse to the potentially detrimental lack of PrP during embryogenesis while its presence is less influential or redundant at later developmental stages.

Dates et versions

hal-03339362 , version 1 (09-09-2021)

Identifiants

Citer

Manal Khalife, Rachel Young, Sead Chadi, Sandrine Le Guillou, Lucas Lefevre, et al.. Transcriptomic Analysis Highlights Time-specific Embryonic Adaptation of Mice to the Lack of PrP. Prion, 2010, 4 (3), Non spécifié. ⟨10.4161/pri.12773⟩. ⟨hal-03339362⟩
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