Accéder directement au contenu Accéder directement à la navigation
Nouvelle interface
Article dans une revue

Synthesis and use of pHEMA microbeads with human EA.hy 926 endothelial cells

Abstract :

Cancer has become a major problem in public health and the resulting bone metastases a worsening factor. Facing it, different strategies have been proposed and mechanisms involved in tumor angiogenesis are being studied. Enhanced permeability retention (EPR) effect is a key step in designing new anticancer drugs. We have prepared poly 2-hydroxyethyl methacrylate (pHEMA) microbeads to target human endothelial EA.hy 926 cells, a cell line derived from human umbilical vein endothelial cells. Microbeads were synthesized by emulsion precipitation method and carried positive or negative charges. EA.hy 926 cells were cultured in 24-well plates and microbeads were deposited on cells at various times. Scanning and transmission electron microscopy, flow cytometry, confocal microscopy, and three-dimensional (3D) reconstruction were used to characterize microbeads and their location outside and inside cells. Microbeads were uptaken by endothelial cells with a better internalization for negatively charged microbeads. 3D reconstruction of confocal optical sections clearly evidenced the uptake and internalization of microbeads by endothelial cells. pHEMA microbeads could represent potential drug carrier in tumor model of metastases.

Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : lundi 20 septembre 2021 - 15:18:44
Dernière modification le : lundi 14 novembre 2022 - 01:46:04

Lien texte intégral




Hervé Nyangoga, Teodora Zecheru, Robert Filmon, Michel-Félix Baslé, Corneliu Cincu, et al.. Synthesis and use of pHEMA microbeads with human EA.hy 926 endothelial cells. Journal of Biomedical Materials Reserach Part B Applied Biomaterials, 2009, 89B (2), pp.501 - 507. ⟨10.1002/jbm.b.31240⟩. ⟨hal-03349472⟩



Consultations de la notice