Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Thiazolidinediones induce osteocyte apoptosis and increase sclerostin expression

Abstract :

Aims  Thiazolidinediones (TZDs) are associated with a higher risk of bone fracture in women compared with men. The aim of the present study was to investigate whether TZDs could influence osteocyte behaviour and contribute to the skeletal phenotype observed in TZD-treated patients.Methods  The murine MLO-Y4 cell line was used as a source of osteocytes. These cells were cultured for 24 h with 0, 10−8 m, 10−7 m, 10−6 m, 10−5 m or 10−4 m of pioglitazone, rosiglitazone or troglitazone in the presence or absence of 17β-oestradiol. The extent of osteocyte apoptosis was assessed, as was the expression of the bone formation inhibitor sclerostin and receptor activator for nuclear factor κB ligand (RANKL) also.Results  In the absence of 17β-oestradiol, pioglitazone, rosiglitazone and troglitazone induced osteocyte apoptosis dose-dependently even at the lowest concentration of 10−8 m. Furthermore, the expression of sclerostin but not RANKL was significantly increased in TZD-treated cultures compared with untreated cultures. The presence of 17β-oestradiol significantly reduced TZD-induced osteocyte apoptosis and also sclerostin up-regulation.Conclusions  These findings therefore raise the potential concern of using TZDs in post-menopausal women where the lack of oestrogen would not prevent osteocyte apoptosis and sclerostin up-regulation and may aggravate the reduction in bone mass in these patients.

Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03349506
Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : lundi 20 septembre 2021 - 15:28:52
Dernière modification le : mercredi 19 janvier 2022 - 16:26:02
Archivage à long terme le : : mardi 21 décembre 2021 - 19:02:12

Fichier

2010-diabeticmedicine.pdf
Accord explicite pour ce dépôt

Identifiants

Collections

Citation

Guillaume Mabilleau, Aleksandra Mieczkowska, M.-E. Edmonds. Thiazolidinediones induce osteocyte apoptosis and increase sclerostin expression. Diabetic Medicine, 2010, 27 (8), pp.925-932. ⟨10.1111/j.1464-5491.2010.03048.x⟩. ⟨hal-03349506⟩

Partager

Métriques

Consultations de la notice

4

Téléchargements de fichiers

19