Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation

Abstract :

Human blood plasma contains a plethora of proteins, encompassing not only proteins that have plasma-based functionalities, but also possibly every other form of low concentrated human proteins. As it circulates through the tissues, the plasma picks up proteins that are released from their origin due to physiological events such as tissue remodeling and cell death. Specific disease processes or tumors are often characterized by plasma “signatures,” which may become obvious via changes in the plasma proteome profile, for example, through over expression of proteins. However, the wide dynamic range of proteins present in plasma makes their analysis very challenging, because high-abundance proteins tend to mask those of lower abundance. In the present study, we used a strategy combining iTRAQ as a reagent which improved the peptide ionization and peptide OFFGEL fractionation that has already been shown, in our previous research, to improve the proteome coverage of cellular extracts. Two prefractioning methods were compared: immunodepletion and a bead-based library of combinatorial hexapeptide technology. Our data suggested that both methods were complementary, with regard to the number of identified proteins. iTRAQ labelling, in association with OFFGEL fractionation, allowed more than 300 different proteins to be characterized from 400  microgramme of plasma proteins.

Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : mercredi 20 octobre 2021 - 10:45:10
Dernière modification le : vendredi 10 décembre 2021 - 03:56:10
Archivage à long terme le : : vendredi 21 janvier 2022 - 19:28:18


Accord explicite pour ce dépôt




Emilie Ernoult, Anthony Bourreau, Erick Gamelin, Catherine Guette. A Proteomic Approach for Plasma Biomarker Discovery with iTRAQ Labelling and OFFGEL Fractionation. BioMed Research International , Hindawi Publishing Corporation, 2009, 2010, pp.927917. ⟨10.1155/2010/927917⟩. ⟨hal-03388016⟩



Consultations de la notice


Téléchargements de fichiers