Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Two-step OFFGEL approach for effective peptide separation compatible with iTRAQ labeling

Abstract :

Shotgun proteomic analyses are increasingly becoming methods of choice for complex samples. The development of effective methods for fractionating peptides to reduce the complexity of the sample before mass analysis is a key point in this strategy. The OFFGEL technology has recently become a tool of choice in proteomic analysis at peptide level. This OFFGEL electrophoresis (OGE) approach allows the in-solution separation of peptides from various biological sources by isoelectric focusing in highly resolved 24 fractions. It was also demonstrated that OGE technology is a filtering tool for pI-based validation of peptide identification. As peptide OGE is compatible with iTRAQ labeling, OGE is finding valuable applications in quantitative proteomics as well. The aim of this study is to explain a new 2D-OGE approach that improves the proteomic coverage of complex mixtures such as colorectal cell line lysates, and which is compatible with iTRAQ labeling.

Keywords : iTRAQ OFFGEL OGE technology
Type de document :
Article dans une revue
Liste complète des métadonnées
Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : jeudi 21 octobre 2021 - 10:22:25
Dernière modification le : mercredi 10 novembre 2021 - 15:32:04

Lien texte intégral




Alexis Rideau, Damien Besson, Alice Boissard, Olivier Coqueret, Catherine Guette. Two-step OFFGEL approach for effective peptide separation compatible with iTRAQ labeling. Proteomics, 2013, 13 (22), pp.3261 - 3266. ⟨10.1002/pmic.201300161⟩. ⟨hal-03389738⟩



Consultations de la notice