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Axonal regeneration is compromised in NFH-LacZ transgenic mice but not in NFH-GFP mice

Abstract :

To investigate neurofilament (NF) dynamics during the cytoskeleton reorganization in regenerating axons, and their electrophysiological and histological consequences, we used two transgenic lines of mice: neurofilament high (NFH)-LacZ and NFH-green fluorescent protein (GFP). In NFH-LacZ mice, NFs are retained in cell bodies and deficient in axons (Eyer and Peterson, 1994), while in NFH-GFP mice the fluorescent fusion protein is normally transported along axons (Letournel et al., 2006). Following a crush of the sciatic nerve, conduction recovery in NFH-GFP mice is similar to wild-type (wt) mice, but it is reduced in NFH-LacZ mice. Moreover, changes of axonal calibres following regeneration are similar between NFH-GFP and wt mice, but they are systematically reduced in NFH-LacZ mice. Finally, the axonal transport of NFH-GFP fusion protein and NFs is re-initiated after the crush as evidenced by the fluorescent and immunolabelling of axons distal from the crushed point, but NFs and the fusion protein are not transported along axons during regeneration in NFH-LacZ mice. Together, these results argue that the absence of axonal NFs in NFH-LacZ mice compromises the axonal regeneration, and that the NFH-GFP reporter fusion protein represents an efficient model to evaluate the NF dynamics during axonal regeneration.

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Soumis le : mardi 26 octobre 2021 - 10:07:09
Dernière modification le : mercredi 27 octobre 2021 - 04:04:36




Julien Cassereau, Guillaume Nicolas, Pierre Lonchampt, Maud Pinier, Annick Barthelaix, et al.. Axonal regeneration is compromised in NFH-LacZ transgenic mice but not in NFH-GFP mice. Neuroscience, Elsevier - International Brain Research Organization, 2013, 228, pp.101 - 108. ⟨10.1016/j.neuroscience.2012.10.011⟩. ⟨hal-03403300⟩



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