Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Axoskeletal proteins prevent oligodendrocyte from toxic injury by upregulating survival, proliferation, and differentiation in vitro

Abstract :

Neurofilaments (NF) are detected in the cerebrospinal fluid of multiple sclerosis (MS) patients, and their concentration correlates with disease severity. We recently demonstrated that NF and co-isolated proteins increase the proliferation and differentiation of oligodendrocytes (OL) in vitro. If these proteins are released in the extracellular environment in MS, they might then regulate remyelination by OL. To test this hypothesis we took advantage of a paradigm of OL toxic injury using lysophosphatidyl choline (LPC), which decreases proliferation and differentiation of surviving cells, and destroys myelin-like membranes. In OL cultures that have been treated with LPC, NF fractions as well as tubulin (TUB) significantly improved recovery: the number of OL progenitors (OLP, A2B5+ cells) increased by 100% and their proliferation by 200%, whereas differentiated (CNP+) and mature (MBP+) cells increased by 150% compared to cultures treated with LPC alone. When added at the time of LPC treatment, NF and TUB protected OL from LPC toxicity; they increased OLP by 90%, as well as the number of CNP+ and MBP+ OL by 65–110%, respectively, compared to cultures treated only with LPC. These effects were specific since irrelevant proteins (actin, skin proteins) were ineffective. This demonstrates that NF and TUB protect OL and increase OLP proliferation, as well as their survival, when challenged with LPC, without delaying differentiation and maturation in vitro. Thus, NF and TUB delivered following axonal damage in MS could participate in the regulation of remyelination through this process.

Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03403303
Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : mardi 26 octobre 2021 - 10:07:15
Dernière modification le : mardi 26 octobre 2021 - 10:07:17

Identifiants

Collections

Citation

Catherine Fressinaud, Joël Eyer. Axoskeletal proteins prevent oligodendrocyte from toxic injury by upregulating survival, proliferation, and differentiation in vitro. Neurochemistry International, Elsevier, 2013, 62 (3), pp.306 - 313. ⟨10.1016/j.neuint.2012.12.012⟩. ⟨hal-03403303⟩

Partager

Métriques

Consultations de la notice

6