Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity

Abstract :

BACKGROUND: Sirtuins (SIRT1-7) are a family of NAD-dependent deacetylases and/or ADP-ribosyltransferases that are involved in metabolism, stress responses and longevity. SIRT3 is localized to mitochondria, where it deacetylates and activates a number of enzymes involved in fuel oxidation and energy production. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we performed a proteomic screen to identify SIRT3 interacting proteins and identified several subunits of complex II and V of the electron transport chain. Two subunits of complex II (also known as succinate dehydrogenase, or SDH), SDHA and SDHB, interacted specifically with SIRT3. Using mass spectrometry, we identified 13 acetylation sites on SDHA, including six novel acetylated residues. SDHA is hyperacetylated in SIRT3 KO mice and SIRT3 directly deacetylates SDHA in a NAD-dependent manner. Finally, we found that SIRT3 regulates SDH activity both in cells and in murine brown adipose tissue. CONCLUSIONS/SIGNIFICANCE: Our study identifies SDHA as a binding partner and substrate for SIRT3 deacetylase activity. SIRT3 loss results in decreased SDH enzyme activity, suggesting that SIRT3 may be an important physiological regulator of SDH activity.

Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal.univ-angers.fr/hal-03408515
Contributeur : Okina Univ Angers Connectez-vous pour contacter le contributeur
Soumis le : vendredi 29 octobre 2021 - 10:51:36
Dernière modification le : samedi 8 janvier 2022 - 03:03:14
Archivage à long terme le : : lundi 31 janvier 2022 - 09:38:47

Fichier

journal.pone_.0023295.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Lydia Finley, Wilhelm Haas, Valérie Desquiret-Dumas, Douglas Wallace, Vincent Procaccio, et al.. Succinate dehydrogenase is a direct target of sirtuin 3 deacetylase activity. PLoS ONE, Public Library of Science, 2011, 6 (8), pp.e23295. ⟨10.1371/journal.pone.0023295⟩. ⟨hal-03408515⟩

Partager

Métriques

Consultations de la notice

3

Téléchargements de fichiers

11